| Acronym | Definition |
|---|
| USF | University of South Florida |
| USF | Universal Service Fund (often part of phone bill in US) |
| USF | University of San Francisco |
| USF | University of Sioux Falls |
| USF | University of Saint Francis (Fort Wayne, IN) |
| USF | University of St. Francis (Joliet, IL) |
| USF | Universal Studios Florida (theme park) |
| USF | US Foodservice (various locations) |
| USF | Universal Subtitle Format |
| USF | US Fleet (US Navy) |
| USF | Upstream Stimulatory Factor |
| USF | Universal Service Fee |
| USF | United States Flag |
| USF | Utah Shakespearean Festival |
| USF | Und So Fort (German: And So Forth) |
| USF | Usable Square Feet (real estate) |
| USF | Unit Set Fielding (US Army methed for distribution of equipment to units) |
| USF | Union Suisse des Fiduciaires (French: Swiss Fiduciary Association) |
| USF | Union Station Foundation (California) |
| USF | Unified Sportsmen of Florida |
| USF | Uplink State Flag |
| USF | Ungdommens Sjakkforbund (Norwegian Youth Chess Federation) |
| USF | United Space Federation (Star Trek gaming) |
| USF | Universal Server Farm |
| USF | Unterhaltungs Software Forum |
| USF | Union Sportive Fontenaysienne (French sports organization) |
| USF | Ultrasonic Fatigue |
| USF | United Scleroderma Foundation |
| USF | Unilever Superannuation Fund (mutual fund) |
| USF | United States Freightways |
| USF | Union Sportive Ferrièroise (French: Ferrièroise Sports Union; La Ferrière, France) |
| USF | Undefined State Fault |