| Acronym | Definition |
|---|
| TLO | Texas Legislature Online |
| TLO | Threat Level Orange |
| TLO | True Living Organics (horticulture) |
| TLO | The Lucky One (book and film) |
| TLO | Tenant Liaison Officer (various companies; UK) |
| TLO | Trichotillomania Learning Center (Santa Cruz, CA) |
| TLO | The Last Olympian (novel) |
| TLO | Traveller Liaison Officer (UK) |
| TLO | The Little One |
| TLO | The Last One (Consultants) |
| TLO | Thematic Learning Objective |
| TLO | Technology Licensing Organization (Japan) |
| TLO | Twitter Link Only (social media) |
| TLO | Terminal Learning Objective |
| TLO | Total Loss Only |
| TLO | The Lancet Oncology (publication) |
| TLO | Terrorism Liaison Officer |
| TLO | Time-Limited Offer |
| TLO | Tribal Liaison Office (Afghanistan) |
| TLO | Time and Labor Online (software) |
| TLO | Technical Liaison Officer |
| TLO | Top-Level Object (object oriented programming) |
| TLO | Toulouse Les Orgues (French: The Toulouse Organs; music festival) |
| TLO | Temporary Lay-Off |
| TLO | Technical Liaison Office (Telcordia) |
| TLO | Task-Level Organization |
| TLO | Thoracolumbar Orthosis |
| TLO | The List Online (subscription based resource) |
| TLO | Three Letter Organization |
| TLO | Train Load-Out (area where coal product is loaded onto train) |