| Acronym | Definition |
|---|
| TLSA | TurboLinux Security Advisory |
| TLSA | TurboLinux Security Announcement |
| TLSA | Torso-Limb Suit Assembly (NASA) |
| TLSA | Tasmanian Learning and Skills Authority |
| TLSA | Teachers League of South Africa |
| TLSA | Teshekpuk Lake Special Area |
| TLSA | Transmission Line Siting Act (Florida) |
| TLSA | Tennessee Land Stewardship Association |
| TLSA | Twin Lakes Soccer Association (Mountain Home, Arkansas) |
| TLSA | Truck Lease and Service Agreement (Ryder Trucks) |
| TLSA | Time Labeled Scheduling Automaton |
| TLSA | Three-Layered Services Application |
| TLSA | Traveller Learning Support Assistant (UK) |
| TLSA | Tunable Laser Subassembly |
| TLSA | Tunable Laser Spectrum Analyzer |
| TLSA | transitional lands study area |
| TLSA | Transmission Line Surge Arrester |
| TLSA | Tallahassee Legal Secretaries Association (Florida) |
| TLSA | Tucson Legal Support Association (Arizona) |
| TLSA | Transparent Line Sharing Adapter |
| TLSA | THINQ Learning Solution Alliance |
| TLSA | Texas Lutheran Schools Association |
| TLSA | Timberline Lodge and Ski Area (Oregon) |