| Acronym | Definition |
|---|
| AFO | Ankle-Foot Orthosis |
| AFO | Animal Feeding Operation(s) |
| AFO | Aquatic Facility Operator (various locations) |
| AFO | Association Française des Orchestres (French: French Association of Orchestras) |
| AFO | Advanced File Organizer |
| AFO | Association Française d'Ostéopathie (French: French Association of Osteopathy) |
| AFO | Air Force One (The Movie) |
| AFO | Arellano-Felix Organization (drug trafficking cartel) |
| AFO | Algar-Flynn-Oyamada (chemical reaction) |
| AFO | Alien Front Online (Sega video game) |
| AFO | Admiralty Fleet Order |
| AFO | Academy of Fixed Orthodontics (various locations) |
| AFO | Air Force Ones (Nike shoes) |
| AFO | Association Française d'Orthoptique (French: French Orthoptic Association; est. 1965) |
| AFO | Authorised Firearms Officer (UK police) |
| AFO | Advanced Furniture Outfitters (Bryan, OH) |
| AFO | Armed Forces Only |
| AFO | Advanced Force Operations (US DoD) |
| AFO | Additional Forms |
| AFO | Air Failure Open (valve operation mode) |
| AFO | Alzheimer's Family Organization (Florida) |
| AFO | Axial Force Only (engineering) |
| AFO | Awaiting Further Occurrence |
| AFO | Auxiliary Fuel Oil |
| AFO | Announced Flight Opportunity |
| AFO | Announcement of Flight Opportunities |
| AFO | Anti-Fascist Organization |
| AFO | Advanced File Organizer (software) |
| AFO | Accounting & Finance Office/r |
| AFO | Audio Frequency Overlay (train location technology) |
| AFO | Alaska Field Office |
| AFO | Agency Field Office |
| AFO | Aero Force One (Aerosmith fan club) |
| AFO | Agencia Filatelica Oficial (Spanish: Official Philatelic Agency, Barcelona) |
| AFO | Away from Office |
| AFO | Available for Obligations |
| AFO | Aircraft Flight Operations (job title) |
| AFO | Army Finance Officer |
| AFO | Alternate Fridays Off (work schedule) |