| Acronym | Definition |
|---|
| USL | United Soccer Leagues |
| USL | Utah State Library |
| USL | Uterosacral Ligament (anatomy) |
| USL | Unità Sanitarie Locali (Italian: Local Sanitary Unity) |
| USL | Union Sportive de Luzenac (French: Sports Union of Luzenac; Luzenac, France) |
| USL | Underwater Sound Laboratory (US Navy) |
| USL | Underdog Sports Leagues (Seattle, WA) |
| USL | Universal Static Line |
| USL | United Spirits Limited (India) |
| USL | University of Southwestern Louisiana |
| USL | UNIX System Laboratories |
| USL | Upper Specification Limit |
| USL | United States 12 Month Oil Fund, LP (stock symbol) |
| USL | Unskilled Labor |
| USL | University of St. Louis (Tuguegarao City, Philippines) |
| USL | Universal Sign Language |
| USL | Unorthodox Scripting Language (computer programming) |
| USL | Unlimited Site License (software agreement) |
| USL | User System Language |
| USL | User Services Layer |
| USL | Up Stage Left |
| USL | Universal Static Line (US Army) |
| USL | Union Sportive Lavalloise (French sports organization) |
| USL | Union des Sociétés Lausannoises (French: Union of Societies, Lusanne; Switzerland) |
| USL | United States Laboratory |
| USL | University School of the Lowcountry (Mt. Pleasant, SC) |
| USL | Union Sportive Luzechoise (French sports organization) |
| USL | Underwater Sound Lab (US Navy, Fort Trumbull, New London, CT) |
| USL | Under Slung Load (aviation) |
| USL | User Segmented Library |
| USL | Unites States Lines, Inc |
| USL | Upper Statistical Limit |