| Acronym | Definition |
|---|
| UNO | University of New Orleans (Louisiana) |
| UNO | University of Nebraska at Omaha (Omaha, NE) |
| UNO | United Nations Office (various locations) |
| UNO | United Nations Organization |
| UNO | Universal Network Objects |
| UNO | Unary Operator |
| UNO | United Nations Orchestra |
| UNO | Unit Number (US DoD) |
| UNO | United Neighborhood Organization |
| UNO | Unless Noted Otherwise |
| UNO | Unniloctium (hassium, element 108) |
| UNO | Unión Nacional Opositora |
| UNO | Under, Not Over (various locations) |
| UNO | Unidentified Noisy Object (band) |
| UNO | Urology Nurses Online (journal) |
| UNO | Universal Networked Objects |
| UNO | Unrestricted Natural Orbital |
| UNO | Unidad Nacionalista Organizada (Spanish: Organized Nationalist Union; Guatemala) |
| UNO | Universal Network Operations |
| UNO | Unified National O-Ring |
| UNO | Ultimate Neutrino Observatory |