| Acronym | Definition |
|---|
| VLS | Vertical Launch System |
| VLS | Virtual Linux Station |
| VLS | Versata Logic Server |
| VLS | Variable Length Structure |
| VLS | Virtual Library Systems |
| VLS | Video Lan Server |
| VLS | Virtual Lan Link State |
| VLS | Vulvar Lichen Sclerosus (dermatosis) |
| VLS | Vapor-Liquid-Solid |
| VLS | Virtual Library System |
| VLS | Vinyl Single |
| VLS | Vascular Leak Syndrome (medical disorder) |
| VLS | Virtual Learning Space |
| VLS | Very Long Shot |
| VLS | Vélos en Libre Service (French: free bicycling service) |
| VLS | Fraunhofer-Verbund Life Sciences (Germany) |
| VLS | Valk Last Slot (Rubik's Cube) |
| VLS | Variable Load Space (forestry vehicle) |
| VLS | Volunteer Life Saving Corps |
| VLS | Vodka, Lime and Soda |
| VLS | Vertical Lay System (subsea production wells) |
| VLS | Victory Lane Speedway (Canada) |
| VLS | Vandenberg Launch and Landing Site |
| VLS | Vehicle Lease and Service Limited (UK) |
| VLS | Variable Low Speed |
| VLS | Valley Library Consortium (Saginaw, Michigan) |