| Acronym | Definition |
|---|
| BOT | Bottom |
| BOT | Back on Topic |
| BOT | Be on Time |
| BOT | Bank of Thailand |
| BOT | Robot (especially a software agent) |
| BOT | Build-Operate-Transfer (project outsourcing; information technology delivery model) |
| BOT | Bottle |
| BOT | Bought |
| BOT | Board of Trade |
| BOT | Botany |
| BOT | Botswana |
| BOT | Board of Trustees |
| BOT | Block of Time (phone plan) |
| BOT | Bank of Tanzania (est. 1965) |
| BOT | Back on Track |
| BOT | Band of Thieves |
| BOT | Books On Tape (audio books) |
| BOT | Bureau of Treasury (Philippines) |
| BOT | Base of Tongue |
| BOT | Base of Tail |
| BOT | Best Of Times |
| BOT | Board of Occupational Therapy (various locations) |
| BOT | Balance of Trade |
| BOT | Bulk Only Transport |
| BOT | Robot |
| BOT | Build Operate and Transfer |
| BOT | Buttons over Titles |
| BOT | Burton-On-Trent (UK town) |
| BOT | Beginning Of Tape |
| BOT | Bottom-Up Approach |
| BOT | Basal-Supported Oral Therapy (diabetes) |
| BOT | Beans on Toast |
| BOT | Baker Oil Tools |
| BOT | Buoni Ordinari del Tesoro (Italian: Treasury Bill) |
| BOT | Back Office Transformation (various companies) |
| BOT | Bomb on Target (US DoD) |
| BOT | Birds of Tokyo (band) |
| BOT | Air Botswana (ICAO code) |
| BOT | British Overseas Territory |
| BOT | Bastion of Thunder (gaming) |
| BOT | Basic Officer Training |
| BOT | Beginning of Track |
| BOT | Bachelor of Occupational Therapy |
| BOT | Boots of Travel (gaming) |
| BOT | Beginning Of Table |
| BOT | Boom Operator Trainer (US DoD) |
| BOT | Bearings-Only Tracking |
| BOT | Buy Operate Transfer |
| BOT | Brussels Overleg Thuiszorg (Dutch: Brussels Homecare Consultation; Brussels, Belgium) |
| BOT | Broadcast Online Television |
| BOT | Beginning Of Transmission |
| BOT | Bottom of Tape |
| BOT | Burst On Target |
| BOT | Business Operations Team |
| BOT | Basic Orientation Training |
| BOT | Beginning Of Tour |
| BOT | Biotronic Operational Telecommunicator (Transformers cartoon) |
| BOT | Blanket Open Test Assembly |
| BOT | Bill of Operation & Transfer |
| BOT | Broad Operational Task |