| Acronym | Definition |
|---|
| DO | Director of Operations |
| DO | Data Object |
| DO | Doctor of Osteopathy |
| DO | Day Off |
| DO | Data Out |
| DO | Due Out |
| DO | Delivery Order |
| DO | District Office |
| DO | Driver Only (train operated by the driver only) |
| DO | Direct Object |
| DO | Duty Officer |
| DO | Digital Output |
| DO | Design Overview |
| DO | Development Officer |
| DO | Director Officer |
| DO | Dominican Republic |
| DO | Disorder |
| DO | Desk Officer (NYPD) |
| DO | Dominica |
| DO | Diário Oficial (Official Gazette; Brazil) |
| DO | Distributed Object |
| DO | District Officer |
| DO | Dissolved Oxygen |
| DO | Ditto |
| DO | Division Officer |
| DO | Dornier (German aircraft manufacturer) |
| DO | Drawing Office |
| DO | Doctor of Osteopathic Medicine |
| DO | Detention Officer |
| DO | Designated Official |
| DO | Design Objective |
| DO | Discrete Output |
| DO | Doctor of Obstetrics |
| DO | Distributed Operations |
| DO | Deportation Order |
| DO | Disaster Operations (US FEMA) |
| DO | Departmental Offices |
| DO | Denominacion de Origen (general identification for Spanish wines) |
| DO | Disbursing Officer |
| DO | Operations Directorate (NIMA) |
| DO | Dispensing Optician |
| DO | Directorate of Operations (US CIA) |
| DO | Division Operator |
| DO | Digital Orthophoto (FEMA) |
| DO | Divisional Officer |
| DO | Ombudsmannen mot Etnisk Diskriminering (the Swedish national ombudsman for issues on ethnic discrimination) |
| DO | Diffractive Optics (camera lenses technology) |
| DO | Determination Order (workers compensation claims) |
| DO | Defense Order |
| DO | Dommages Ouvrage (French: Work Damage) |
| DO | Desktop Originator |
| DO | Disciplined Oscillator |
| DO | District Overseer (Jehovah's Witnesses) |
| DO | Disbursement Order |
| DO | Operational Dependability |
| DO | Drift Orbit |
| DO | DCS/Operations |
| DO | Donk Out (poker) |
| DO | Disbursing Order |
| DO | Diode Outline |
| DO | Disto-Occlusal (dentistry) |
| DO | Distortion Offset |
| DO | Dark One (Wheel of Time series) |
| DO | Diatomic Orbital |
| DO | Doctor of Otolaryngology |
| DO | Defined Order |
| DO | US Duty Officer |
| DO | Daily Orange (Syracuse University student newspaper; New York) |
| DO | Dawson and Oberman (plasmas) |