| Acronym | Definition |
|---|
| DLO | Digital Lifestyle Outfitters (Charleston, SC) |
| DLO | Document Library Object |
| DLO | Dynamically Loaded Object |
| DLO | Dead Letter Office |
| DLO | Defence Logistics Organisation |
| DLO | Desktop and Laptop Option (Symantec backup products) |
| DLO | Document Library Object (computing) |
| DLO | Dienst Landbouwkundig Onderzoek (Netherlands) |
| DLO | Direct Labour Organisation (UK; local authority trading departments) |
| DLO | Day Light Opening |
| DLO | Departmental Liaison Officer |
| DLO | Data Line Occupied |
| DLO | Double Layout (gymnastics) |
| DLO | Defense Liaison Office |
| DLO | Difference of Longitude |
| DLO | District Legal Office (US Navy) |
| DLO | Diploma in Otorhinolaryngology |
| DLO | Data Link Occupied |
| DLO | Defense Law & Order |
| DLO | Disclosed Limit Order (stock market) |
| DLO | Diesel Lubricating Oil Service and Transfer |
| DLO | Dienst Lichamelijke Opvoeding (Dutch) |
| DLO | Desired Learning Objective |
| DLO | Division de Logistique Opérationnelle (French: Division of Logistical Operations) |
| DLO | Detachment Liaison Officer |
| DLO | Données Logistiques Optimisées (French: Optimized Logistics Data) |
| DLO | Deck Low |
| DLO | Domestic Light Orchestra (England, UK) |