DOTA
| Acronym | Definition |
|---|---|
| DOTA | Defense of the Ancients (gaming) |
| DOTA | Depending on the Artist (trope) |
| DOTA | Doscientos Ocho Transporte Automotor (Buenos Aires bus line; Buenos Aires, Argentina) |
| DOTA | Download over the Air (Wavecom Wireless) |
| DOTA | Declaration of the Americas on Diabetes |
| DOTA | Département d'Optique Théorique et Appliquée (French: Theoretical and Applied Optics Department) |
| DOTA | Delaware Occupational Therapy Association |
| DOTA | Department of Transportation, Airports Division (Hawaii) |