| Acronym | Definition |
|---|
| EOF | End Of File |
| EOF | Electroosmotic Flow |
| EOF | Empirical Orthogonal Functions |
| EOF | End of Frame |
| EOF | Educational Opportunity Fund (various organizations) |
| EOF | Echoes of Faydwer (game) |
| EOF | Evaluated Optional Feature |
| EOF | Ethernet over Fiber |
| EOF | Enterprise Objects Frameworks |
| EOF | Executive Office Furniture |
| EOF | Escalation of Force |
| EOF | Emerging Opportunities Fund (various organizations) |
| EOF | Église Orthodoxe Française (French: French Orthodox Church) |
| EOF | Electronic Order Form |
| EOF | École Ouverte Francophone (French educational project) |
| EOF | Electronic Order Fulfillment |
| EOF | Experimenters' Operations Facility |
| EOF | Empire Opportunity Fund |
| EOF | Eyes of Fire (movie) |
| EOF | Enterprise Object Frameworks |
| EOF | Eternal Order of Fighters (Quest for Glory' game series) |
| EOF | Expansile Osteolysis, Familial |
| EOF | Ethnikos Organismos Farmakon (Greek National Organization for Medicines) |
| EOF | Essential Operating Facility |
| EOF | Emergency Operating/Operations Facility |
| EOF | Executives on Fire (band) |