| Acronym | Definition |
|---|
| GLS | Galveston (Amtrak station code; Galveston, TX) |
| GLS | General Logistics Systems |
| GLS | Graduate Liberal Studies (various schools) |
| GLS | Generalized Least Squares |
| GLS | Grand Luxe Super |
| GLS | Glossary |
| GLS | Global Link Services |
| GLS | Global Leadership Series (various locations) |
| GLS | Great Lake Swimmers (band) |
| GLS | Gays Lesbicas e Simpatizantes |
| GLS | General Legal Services |
| GLS | Glide Slope |
| GLS | Glass Laminating Solutions (DuPont) |
| GLS | GPS Landing System |
| GLS | General Lighting Service |
| GLS | Gallium Lanthanum Sulfide Glass |
| GLS | Gay and Lesbian Sierrans |
| GLS | Guy L Steele (Jargon File author) |
| GLS | Grace Lutheran School |
| GLS | Global Labor Strategies |
| GLS | Ground Launch Sequencer (NASA) |
| GLS | Grid Location Service (routing) |
| GLS | GNSS Landing System |
| GLS | Global Logistics Specialist |
| GLS | General Ledger System |
| GLS | Galveston, TX, USA - Scholes Field (Airport Code) |
| GLS | Global Logistics System |
| GLS | Greg Larson Sports (Texas) |
| GLS | Global Listing Service (real estate) |
| GLS | Global Life Sciences, Inc. |
| GLS | Global Lifescience Solutions |
| GLS | Guest Lecturer Series (various organizations) |
| GLS | Good Luck Selling |
| GLS | Great Lord Scepter (gaming) |
| GLS | GoodLink Server |
| GLS | Georgia Local Section |
| GLS | Gust Load Alleviation |
| GLS | Generator Load Sensor |
| GLS | Global Logical Schema |
| GLS | Gnome Land Security (World of Warcraft Alliance guild) |
| GLS | Government Launch Services |
| GLS | General Line School |
| GLS | Grand Luxo Super |
| GLS | Gate Lock Switch |