| Acronym | Definition |
|---|
| HLS | Hills |
| HLS | Harvard Law School |
| HLS | High-Level Synthesis |
| HLS | Homeland Security |
| HLS | Huntingdon Life Sciences (research laboratory, Cambridgeshire, England) |
| HLS | High Level Structure (International Organization for Standardization) |
| HLS | Higher Level Stewardship (UK; agriculture) |
| HLS | Healthcare and Life Sciences |
| HLS | Helicopter Landing Site |
| HLS | Hue Lightning Saturation |
| HLS | High Line Super |
| HLS | Home Location Server |
| HLS | High Level Service |
| HLS | Higher Level Skills |
| HLS | Helicopter Landing System |
| HLS | Hue, Lightness, Saturation (color model, aka HVS) |
| HLS | Hard Luck Story |
| HLS | Hispanic Linguistics Symposium (various locations) |
| HLS | Hawkes Learning Systems (software) |
| HLS | Hurricane Local Statement (US FEMA) |
| HLS | Heat, Light and Sound (various organizations) |
| HLS | Health Level Seven (healthcare) |
| HLS | Handheld Laser Scanner |
| HLS | Human Lineage Specific (genetics) |
| HLS | High Level Sports |
| HLS | Hot Lime Softener (water softener) |
| HLS | Healthy Living Strategy (Canada) |
| HLS | Heavy Louisiana Sweet (crude oil) |
| HLS | Hawken Locksmith Services (DeKalb, IL) |
| HLS | Healthy Living Seminar |
| HLS | Hook Loop Strap (product feature) |
| HLS | Home Library Service |
| HLS | Halt Line State (Cisco) |
| HLS | Hydraulic Locomotive Shed (Sri Lanka) |
| HLS | Home Loan Servicing |
| HLS | Hot Lava Software |
| HLS | Homeland Support |
| HLS | Hyperbaric Lighting Set |
| HLS | Huge Lawn Software (Denmark) |
| HLS | Hybrid Transmission with Layered Source |
| HLS | Horizontal Layered Schedule |
| HLS | Hysterical Laughter Syndrome |
| HLS | HTTP (Hypertext Transfer Protocol) Live Streaming (Apple) |