| Acronym | Definition |
|---|
| LOT | List of Tables |
| LOT | Leaders of Tomorrow (various organizations) |
| LOT | Polskie Linie Lotnicze (Polish Airlines) |
| LOT | Lack of Talent (band) |
| LOT | Look Over There |
| LOT | Letter of Transmittal |
| LOT | Lyric Opera Theatre (various locations) |
| LOT | Line of Travel (USPS Address Code Element) |
| LOT | Licensed Occupational Therapist |
| LOT | Language of Thought (cognitive hypothesis) |
| LOT | Lockport, Illinois (Airport Code) |
| LOT | Left Occiput Transverse (fetal position) |
| LOT | Lock on Target |
| LOT | Line of Technology |
| LOT | Life Orientation Test |
| LOT | Life Of Type |
| LOT | Landelijke Onderzoekschool Taalwetenschap (Dutch: The Netherlands National Graduate School of Linguistics) |
| LOT | Loss Of Track |
| LOT | Library of Texas |
| LOT | Leak-Off Test |
| LOT | Lapped Orthogonal Transform |
| LOT | Line Output Transformer (electronics) |
| LOT | Liaison and Observation Team |
| LOT | Linear Override Tool (energy production) |
| LOT | Linear Quantizer |
| LOT | Low Observables Technology |
| LOT | Lockout Trunk |
| LOT | Local Office Team |
| LOT | Large Orbiting Telescope |
| LOT | Local Office Training |
| LOT | Lease Operate and Transfer |
| LOT | line-of-travel sequence (US Postal Service routing method) |
| LOT | Limited Operational Test |
| LOT | Lotto-Adecho (Tour de France cycling sponsor) |
| LOT | Load Out Truss |
| LOT | Limited Operation Test |