| Acronym | Definition |
|---|
| MLO | Mortgage Loan Officer |
| MLO | Mortgage Loan Originator (real estate) |
| MLO | My Little One |
| MLO | Mediolateral Oblique (mammography) |
| MLO | Mesorhizobium Loti |
| MLO | Medical Laboratory Observer (journal) |
| MLO | Multiple Level Observation |
| MLO | My Life Organized (website) |
| MLO | Mechanical Lock Out (bicycle wheel suspension) |
| MLO | Maestría en Liderazgo Organizacional (Spanish: Master in Organizational Leadership; Guatemala) |
| MLO | Mauna Loa Observatory |
| MLO | Model Lighting Ordinance (International Dark Sky Association/Illuminating Engineering Society) |
| MLO | Mill Levy Override (Colorado) |
| MLO | Most Likely Outcome |
| MLO | Motor Liciencing Officer (India) |
| MLO | Military Liaison Office |
| MLO | Multi-Layer Organic |
| MLO | Misanthropic Luciferian Order (Satanic cult) |
| MLO | Main-Line Operator (shipping) |
| MLO | Material Liaison Office (US Navy) |
| MLO | Milos, Greece - Milos (Airport Code) |
| MLO | Mutated Learning Object |
| MLO | Main Lug Only |
| MLO | Municipal Lighting Ordinance |
| MLO | Manchurian Local Overprint (Chinese postal stamp) |
| MLO | Meta-Level Organization |
| MLO | Malibu Locals Only (street gang) |
| MLO | Master Lease Option |
| MLO | Material, Labor, and Overhead |
| MLO | Mine-Like Object |
| MLO | Multilobular Osteochondrosarcoma (tumor) |
| MLO | Mapua Lights Organization (Manila, Philippines) |
| MLO | Mechanised Letter Office (UK) |
| MLO | Mode-Locked Oscillator |
| MLO | Ministry of Labour/Ontario |
| MLO | Multilateral Organization |
| MLO | Monolinoleate (glycerolmonolipide used to form reversed phases) |