| Acronym | Definition |
|---|
| NLO | Nonlinear Optics |
| NLO | Non-Linear Optimization (mathematics) |
| NLO | Next-to-Leading Order |
| NLO | National Labor Office |
| NLO | Nobody Likes Onions (website) |
| NLO | Novoe Literaturnoe Obozrenie (Russian: New Literary Observer; journal) |
| NLO | Nasolacrimal Obstruction |
| NLO | Naval Liaison Officer |
| NLO | National Liaison Officer |
| NLO | Network Loadable Objects |
| NLO | Nepoznati Leteci Objekt (Croatian: unidentified flying object) |
| NLO | NETg Learning Object |
| NLO | No Load Operation (computer power supplies) |
| NLO | New Little Odessa (gaming, Half-Life 2) |
| NLO | Nasolacrimal Occlusion |
| NLO | National Lead of Ohio Inc. |
| NLO | No Lazy Offense |