| Acronym | Definition |
|---|
| OLT | One Last Thing |
| OLT | Orthotopic Liver Transplantation |
| OLT | Optical Line Terminal |
| OLT | on Line Training |
| OLT | On Line Taxes, Inc |
| OLT | Online Training |
| OLT | Old Town Transit Center (Amtrak station code; San Diego, CA) |
| OLT | Optical Line Termination |
| OLT | Ostfriesische Lufttransport GmbH (Emden, Germany airline) |
| OLT | Operations Leadership Team (various organizations) |
| OLT | Oberleutnant (German: a military rank) |
| OLT | Office of Learning Technologies (Canada) |
| OLT | Oil Loading Terminal (various companies) |
| OLT | Online Learning and Teaching (Queensland University of Technology, Australia) |
| OLT | On-Line Testing |
| OLT | Object Level Trace |
| OLT | Organización de Lucha por la Tierra (Spanish: Organization of Struggle for Land; Paraguay) |
| OLT | Operation Land Transfer |
| OLT | Owners, Landlords, and Tenants (insurance) |
| OLT | Orlando Lutheran Towers (Orlando, FL) |
| OLT | Overwhelmingly Large Telescope |
| OLT | Order Lead Time (logistics) |
| OLT | Oceania Leadership Team |
| OLT | Optical Leak Test |
| OLT | Observatory Level Test |
| OLT | Optical Layup Template |
| OLT | Only Legal Transitions |
| OLT | Organização de Local de Trabalho (Portuguese: Organization of Work) |
| OLT | Operational Level Task |
| OLT | Osteochondral Lesion of the Tibia |