| Acronym | Definition |
|---|
| RLO | Right to Left Override |
| RLO | Reusable Learning Object |
| RLO | Right-to-Left Override |
| RLO | Rijnlands Lyceum Oegstgeest (school; Oegstgeest, Netherlands) |
| RLO | Real Life Options (learning disability support; est. 1992) |
| RLO | Resident Liaison Officer (UK) |
| RLO | Regional Licensing Office |
| RLO | Rough Layout (art) |
| RLO | Record Layout |
| RLO | Regional Liaison Office |
| RLO | Reserve Liaison Officer |
| RLO | Repairs Liaison Officer (US Navy) |
| RLO | Records Liaison Officer |
| RLO | Restriction of Liberty Order (UK) |
| RLO | Richland Operations (US DOE) |
| RLO | Recovery Level Objective (business continuity management) |
| RLO | Radicaux Libres de l'Oxygène (French: Oxygen Free Radicals) |
| RLO | Real Live Officer |
| RLO | Range Liaison Officer |
| RLO | Result of the Logical Operation (Programmable Logic Controller programming) |
| RLO | Rosa-Luxemburg-Oberschule Berlin (Germany) |
| RLO | Restoration Liaison Officer |
| RLO | Radar Lock-On |
| RLO | Related Learning Opportunity |