| Acronym | Definition |
|---|
| RLS | Release |
| RLS | Restless Leg Syndrome |
| RLS | La Sierra (Amtrak station code; La Sierra, CA) |
| RLS | Real Life Support (various locations) |
| RLS | Robert Louis Stevenson |
| RLS | Rapid Light System (various companies) |
| RLS | Recursive Least Squares (a mathematical algorithm) |
| RLS | Rappresentante Dei Lavoratori Per La Sicurezza (Italian: Representative for Job Safety) |
| RLS | Resource Locator Service |
| RLS | Record Level Sharing |
| RLS | Route Listening Service |
| RLS | Record Level Subscriber |
| RLS | Recursive Least Means Squares |
| RLS | Replica Location Service |
| RLS | Registered Land Surveyor |
| RLS | Reweighted Least Squares (mathematics) |
| RLS | Record-Level Sharing (Data Facility Storage Management Subsystem) |
| RLS | Resource List Server |
| RLS | Resonance Light Scattering (spectroscopy) |
| RLS | Rate Limiting Step |
| RLS | Real Life Soap (Wiki) |
| RLS | Residence Life Staff |
| RLS | Rod Lift System (various companies) |
| RLS | Rural Library Sustainability (project; Bill and Melinda Gates Foundation) |
| RLS | Received Line Signal |
| RLS | Root Lateral Spread |
| RLS | Radiolocation Service |
| RLS | Remote Library Service |
| RLS | Real Life Scenario |
| RLS | Renaissance Lawyer Society |
| RLS | Road Locomotive Society (UK) |
| RLS | Resource Loaded Schedule |
| RLS | Radar Line of Sight |
| RLS | Return to Launch Site (also seen as RTLS) |
| RLS | Rail Load Simulator |
| RLS | Residential Living Supervisor |
| RLS | Residual Life of the System |
| RLS | Royal Leamington Sparklers (cheerleading group; UK) |