| Acronym | Definition |
|---|
| USB | Universal Serial Bus |
| USB | Universidad Simon Bolivar (Venezuela) |
| USB | US Bank |
| USB | Unione Sindacale di Base (Italian: Basic Trade Union) |
| USB | United Soybean Board |
| USB | United Sexy Boys (music group) |
| USB | Union Savings Bank (various locations) |
| USB | University of Stellenbosch Business (South Africa) |
| USB | United Security Bancshares |
| USB | User Support Branch |
| USB | Universal Synchronous Bus |
| USB | Upper Side Band |
| USB | Ultimate Sound Bank |
| USB | Upper Sideband |
| USB | US Beverage (Stamford, CT) |
| USB | Unified S-Band (satellite technology) |
| USB | United Southern Bank (formerly Umatilla State Bank) |
| USB | Urban Service Boundary |
| USB | Unteres Seitenband (German: lower sideband) |
| USB | University of Sistan and Baluchestan (Zahedan, Iran) |
| USB | Unified Security Budget |
| USB | Unsporting Behaviour (soccer) |
| USB | Uniwersytet Stefana Batorego (Polish: Stephen Batory University; former Polish name of Vilnius University) |
| USB | Union of Scientists in Bulgaria (est. 1944) |
| USB | Usage Sensitive Billing |
| USB | Upflow Sludge Bed |
| USB | Ultrasound-Guided Aspiration Biopsy |
| USB | User Sensitive Billing |
| USB | Unicode Subset Bitfield |
| USB | User's Support Branch |
| USB | University At Stonybrook (SUNY) |
| USB | University Star Building |
| USB | Union Sportive de Brillon (French: Brillon Sports Union; Brillon, France) |